Agroinfiltration-based efficient transient protein expression in leguminous plants
نویسندگان
چکیده
منابع مشابه
Transient Expression of Foot and Mouth Disease Virus (FMDV) Coat Protein in Tobacco (Nicotiana tabacom) via Agroinfiltration
Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants. Objective: The main goal of the present study was to eva...
متن کاملThe transient expression of coat protein of Foot and Mouth Disease Virus (FMDV) in spinach (Spinacia oleracea) using Agroinfiltration
Foot and Mouth Disease (FMD) is a very dangerous livestock disease which causes a serious loss in the production of milk and meat. Therefore, producing an effective recombinant subunit vaccine virus this disease is of great importance. Transient gene expression is a valuable tool to reach rapid and acceptable recombinant vaccine. An Agrobacterium-mediated transient gene expression assay was car...
متن کاملtransient expression of foot and mouth disease virus (fmdv) coat protein in tobacco (nicotiana tabacom) via agroinfiltration
background: transient and stable transformation of host plants are the common techniques to produce transgenic plants. however, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. while, transient gene expression is a quick method to produce recombinant proteins in plants. objective: the main goal of the present study was to eval...
متن کاملtransient expression of coat protein of foot and mouth disease virus (fmdv) in alfalfa (medicago sativa) by agroinfiltration
an agrobacterium-mediated transient gene expression assay was carried out in alfalfa (medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of foot and mouth disease virus called vp1. the plant leaves were transformed via agroinfiltration procedure. the presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain ...
متن کاملEnvironment Control to Improve Recombinant Protein Yields in Plants Based on Agrobacterium-Mediated Transient Gene Expression
Agrobacterium-mediated transient expression systems enable plants to produce a wide range of recombinant proteins on a rapid timescale. To achieve economically feasible upstream production and downstream processing, two yield parameters should be considered: (1) recombinant protein content per unit biomass and (2) recombinant protein productivity per unit area-time at the end of the upstream pr...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2019
ISSN: 1342-4580,1347-6114
DOI: 10.5511/plantbiotechnology.19.0220b